Gene Targeting
AssigneeAlgentech SAS
InventorsAlexander Sorokin, Isabelle Malcuit, Florina Vlad
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Abstract
Methods, reagents and compositions for providing more accurate and reliable genetic modification are provided. In particular a nucleic acid encoding a fusion protein comprising an endonuclease domain and a binding domain for an origin of replication is described. Also provided are methods, reagents and compositions for in vivo genetic modification of the genome of a non-animal cell or organism. Furthermore, the present application relates to uses of the said methods, reagents and compositions for introducing desirable traits to non-animal organisms or ameliorating or removing non-desirable traits in these organisms including in the treatment of disease.
Claims19
A nucleic acid encoding a first fusion protein comprising a bacteriophage coat protein and a binding domain for an origin of replication.
A method of modifying the genome of a non-animal organism or cell comprising:
- a. expressing in the cell a nucleic acid encoding a first fusion protein comprising a bacteriophage coat protein and a binding domain for an origin of replication or introducing the first fusion protein into the cell;
- and
- b. expressing in the cell or introducing into the cell a donor nucleic acid molecule comprising an origin of replication.
Dependencies are inferred from the claim text.