MODIFIED AMINOACYL-tRNA SYNTHETASE AND USE THEREOF
AssigneeChugai Seiyaku Kabushiki Kaisha
InventorsAtsushi OHTA, Yusuke YAMAGISHI, Atsushi MATSUO
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Abstract
The present invention provides modified arninoacyl-tRNA synthetases (ARSs) having increased reactivity with N-methyl amino acids compared to natural aminoacyl-tRNA synthetases. The modified aminoacyI-tRNA synthetases according to the present invention can aminoacylate tRNAs with their corresponding N-methyl-substituted amino acids such as N-methyl-phenylalanine, N-methyl-valine, N-methyl-serine, N-methyl-threonine, N-methyl-tryptophan, and N-methyl-leucine more efficiently than natural aminoacyl-tRNA synthetases. The present invention enables a more efficient production of polypeptides containing N-methyl amino acids.
Claims16
1-15. (canceled)
A polypeptide comprising a modified phenylalanyl-tRNA synthetase (PheRS), wherein the PheRS is able to incorporate an N-methyl phenylalanine more efficiently than an original, natural PheRS.
A phenylalanyl-tRNA synthetase (PheRS) modified to enhance an aminoacylation reaction with an N-methyl phenylalanine, wherein the modification comprises at least one amino acid substitution.
Dependencies are inferred from the claim text.