Oligonucleotide compositions and methods of use thereof

A chirally controlled oligonucleotide composition comprising a plurality of oligonucleotides, wherein the oligonucleotides share:

• 1) a common base sequence,
• 2) a common pattern of backbone linkages, and
• 3) a common pattern of backbone chiral centers,
• wherein the composition is enriched, relative to a substantially racemic preparation of oligonucleotides sharing the common base sequence and pattern of backbone linkages, for oligonucleotides of the plurality, and
• wherein:
• oligonucleotides of the plurality each independently comprise a phosphorothioate internucleotidic linkage,
• oligonucleotides of the plurality each independently comprise a non-negatively charged internucleotidic linkages, and
• the base sequence comprises at least 15 contiguous nucleobases of a base sequence that is at least 75% identical or complementary to a base sequence of an USH2A gene or a transcript thereof.