METHODS AND MOLECULES FOR RNA INTERFERENCE (RNAi)

A nucleic acid comprising the nucleic acid sequence 5′-TTCGWWWNNAHHWWCATCCGGN-3′ (SEQ ID NO:

• 1), wherein W is A or T, H is A or T or C, and N is A or T or G or C;
• wherein A is adenine, C is cytosine, G is guanine, T is thymine in DNA or uracil in RNA;
• the nucleic acid is optionally RNA comprising the nucleic acid sequence 5′-UUCGWWWNNAHHWWCAUCCGGN-3′ (SEQ ID NO:
• 2);
• and/or the nucleic acid sequence is optionally selected from any one of SEQ ID NO:
• 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25.

A mammalian cell comprising a DNA encoding a gene of interest operatively linked to a sequence of a heterologous RNAi target site;

• further comprising a DNA encoding an inhibitory RNA that has a complementary region to the heterologous RNAi target site;
• wherein said heterologous RNAi target site has a size of at least 18 nucleotides in length;
• wherein the gene of interest is optionally on an exogenous gene;
• wherein an endogenous variant of the exogenous gene is optionally disrupted.