Magnetic bead, and preparation method therefor and use thereof in nucleic acid extraction
AssigneeNanjing GenScript Biotech Co., Ltd.
InventorsLiwei Sun, Juncheng Liu, Peng Jin, Siwen Liu, Liang Zhou, Suya Zhang
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Abstract
A magnetic bead, and a preparation method therefor and the use thereof in nucleic acid extraction. The preparation method for a magnetic bead comprises: preparing a magnetic core by means of a water-soluble salt of Fe2+ and Fe3+; and subjecting the magnetic core and a silanide to a modification reaction to obtain the magnetic bead, wherein the molar ratio of the addition amount of the used silanide to the total amount of Fe2+ and Fe3+ is greater than or equal to 0.5:1, and the particle size range of the magnetic bead is 0.1-50 μm.
Claims30
A preparation method for a magnetic bead, comprising:
- preparing a magnetic core by means of a water-soluble salt of Fe2+ and Fe3+;
- and
- subjecting the magnetic core and a silanide to a modification reaction to obtain the magnetic bead,
- wherein the molar ratio of the addition amount of the used silanide to the total amount of Fe2+ and Fe3+ is greater than or equal to 0.5:1, preferably (0.5:1)-(4.0:1), and the particle size range of the magnetic bead is 0.1 μm-50 μm.
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A method for extracting a nucleic acid, comprising:
- providing a biological sample containing a free nucleic acid and non-nucleic acid impurities;
- adsorbing the non-nucleic acid impurities to an impurity-removing magnetic bead;
- and
- removing the impurity-removing magnetic bead by adding a magnetic field to obtain a solution comprising the nucleic acid,
- wherein the impurity-removing magnetic bead comprises silicon hydroxyl, carboxyl or a modification group having ion exchange properties;
- wherein the silicon hydroxyl is obtained by silanide modification;
- wherein the silanide comprises at least one of methyl orthosilicate, ethyl orthosilicate, propyl orthosilicate, butyl orthosilicate, alkyltrimethoxysilane, alkyltriethoxysilane, alkyltriisopropylsilane, dialkyldimethoxysilane, dialkyldiethoxysilane, phenyltrimethoxysilane, phenyltriethoxysilane, aminopropyltrimethoxysilane and epoxypropyltrimethoxysilane.
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Dependencies are inferred from the claim text.