Purification of macromolecules
AssigneePhyNexus, Inc.
InventorsDouglas GJERDE, Don APLAON
Life of this patent
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Abstract
The present disclosure provides an automated method for chemical treatment of cells in a mixing chamber, wherein the automatable method comprises providing cells, providing a chemical agent for treating cells, forming a liquid mixture comprising the cells and the chemical agent, and mixing the liquid mixture by providing a back-and-forth liquid flow in the mixing chamber. An automated process for purifying macromolecules, such as plasmids, and an automated system comprising a workstation configured for purifying such macromolecules are also provided.
Claims17
An automated method for chemical treatment of cells in a mixing chamber, wherein the automatable method comprises
- providing a mixing chamber,
- providing cells,
- providing a chemical agent for treating cells,
- combining said cells and said chemical agent in the mixing chamber to form a liquid mixture, and
- providing for mixing of said cells and said chemical agent by providing a back-and-forth liquid flow in the mixing chamber.
An automated process for purifying macromolecules, which process comprises
- providing cells,
- providing a lysis buffering agent,
- providing a precipitation agent,
- forming a liquid mixture comprising the cells and the lysis buffering agent, mixing the liquid mixture by providing a back-and-forth liquid flow in the mixing chamber,
- providing a non-precipitated liquid mixture by adding the precipitation agent to at least a portion of the liquid mixture,
- providing a filtrate by passing at least a portion of the non-precipitated liquid mixture through at least one filter.
-19. (canceled)
Dependencies are inferred from the claim text.