Variant maltopentaose/maltohexaose-forming alpha-amylases
AssigneeDanisco US Inc.
InventorsJonathan LASSILA, Manasi BHATE, Amanda CHAN, Hon Kit CHAN, Chris LEEFLANG, Sandra W. RAMER, Patricia TRAN
Life of this patent
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Abstract
Disclosed are compositions and methods relating to maltopentaose/maltohexaose-forming α-amylases. The variant α-amylases are useful, for example, for starch liquefaction and saccharification, for cleaning starchy stains in laundry, dishwashing, and other applications, for textile processing (e.g., desizing), in animal feed for improving digestibility, and for baking and brewing.
Claims11
A recombinant, non-naturally-occurring variant of a parent alpha-amylase, the variant alpha-amylase having 80% identity to SEQ ID NO:
- 5 and having amino acid substitutions at positions 51 and 125 with respect to SEQ ID NO:
- 5.
A recombinant, non-naturally-occurring variant of a parent alpha-amylase, the variant alpha-amylase having 80% identity to SEQ ID NO:
- 5 and having the amino acid substitution:
- (a) N29Q+T51V+S125R+N227R+S253L+G272E+K319R+S418A
- (b) T51V+S125R+F231L;
- (c) T51V+S125R+N172Q+N227R;
- (d) N029Q+T051V+T244I+S253L+K268R+K319R+S418A;
- (e) E415G;
- or
- with respect to SEQ ID NO:
- 5.
Dependencies are inferred from the claim text.