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AssigneeBgi Shenzhen
InventorsXiaohong ZHANG, Xi LAN, Qingqing XIE, Feng XI, Yue ZHENG, Yuliang DONG, Wenwei ZHANG
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The present invention relates to the field of biotechnology, in particular to a DNA polymerase mutant and the use thereof. Compared with a wild type, the mutant provided in the present invention has a significantly improved DNA polymerization activity, significantly improved affinity to DNA and amplification uniformity; and has good effects in the amplification of multiplex PCR, high-GC templates and complex templates. A hot-start-version DNA polymerase is further prepared by means of using antibodies or chemical modifications, wherein the yield of the DNA polymerase is significantly improved, while the primer dimer is significantly decreased. The prepared mutant hot-start DNA polymerase can be applied to PCR amplification of multiplex PCR, high-GC templates and complex templates.
A DNA polymerase mutant comprising at least one mutation selected from the group consisting of K56Q, A61T and E507K, wherein the mutation is relative to a wild type DNA polymerase and the amino acid sequence of the wild type DNA polymerase is set forth in SEQ ID NO:
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