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US 2025/0051731 A1Published application
Compositions and methods for establishment of bovine-induced pluripotent stem cells
OwnerYoung Tang, Yue Su +3inventors
InventorsYoung Tang, Yue Su, Ling Wang, Xiuchyn Tian, Jiaqi Zhu
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Abstract
The disclosure provides bovine-induced pluripotent stem cells along with compositions and methods for use in producing the same.
Claims22
A cell culture medium comprising a WNT signaling inhibitor and a histone methyltransferase DOT1L inhibitor.
A cell culture medium comprising a WNT signaling inhibitor, a histone methyltransferase DOT1L inhibitor, human leukemia inhibitory factor (LIF), and an adenyl cyclase activator.
A method of producing a bovine-induced pluripotent stem cell, the method comprising:
- a. providing a bovine mesenchymal stem cell, and
- b. inducing overexpression of a plurality of reprogramming factors in the bovine mesenchymal stem cell, wherein the plurality of reprogramming factors comprise lysine-specific demethylase 4A (KDM4A), OCT4, SOX2, KLF4, cMYC, LIN28, and NANOG.
Dependencies are inferred from the claim text.