Cost effective culture media and protocol for human induced pluripotent stem cells
AssigneeNorthwestern Unversity
InventorPaul Burridge
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Abstract
A novel culture media formula that is thoroughly optimized to support high growth rate under low seeding density conditions, require minimal media exchanges, and at low cost, while maintaining differentiation reproducibility is provided. This formula is capable of supporting both human induced pluripotent stem cell (hiPSC) generation and culture for >100 passages. Generation of B8 supplement aliquots suitable for making 100 liters of media is simple for any research lab with basic equipment, with complete bottles of media costing ˜$12 USD per liter. Weekend free hiPSC cell culture methods are possible with this formulation.
Claims21
1-18. (canceled)
A method of expanding a pluripotent cell population, the method comprising culturing the cell population on a surface contacting a chemically defined liquid cell culture medium a first time for at least 2 days, passaging the cell population, culturing the cell population on the surface contacting the chemically defined liquid cell culture medium a second time for at least 2 days, and subjecting the cell population to a monolayer differentiation protocol, wherein the chemically defined liquid cell culture medium comprises a nonzero amount of TGF beta less than 2 ng/ml and a nonzero amount of FGF2 less than 100 ng/ml.
Dependencies are inferred from the claim text.