Dendritic Cell Generator

A method of preparing a dendritic cell vaccine, the method comprising:

• providing a dendritic cell generation system comprising:
• one or more fluid reservoirs;
• a pump operably coupled to the one or more fluid reservoirs;
• and
• a cell culture chamber fluidically coupled to the one or more fluid reservoirs, wherein the cell culture chamber comprises a fluidic channel having an inlet, an outlet, and a monocyte binding substrate;
• introducing a liquid sample comprising monocytes of a subject into the fluidic channel of the cell culture chamber via the inlet, whereby at least a portion of said monocytes bind to the monocyte binding substrate of the cell culture chamber;
• flowing a dendritic cell differentiation medium into the cell culture chamber to perfuse the monocytes, whereby at least a portion of the bound monocytes differentiate into dendritic cells and detach from the monocyte-binding substrate as mature dendritic cells;
• and
• exposing the mature dendritic cells to one or more antigens to thereby activate the mature dendritic cells.