Egg opener, ex ovo tumor xenograft method, therapeutic compound assay, therapeutic compound testing method via ex ovo quail egg xenograft assay

A method for preparing an ex ovo tumor xenograft comprising the steps of:

• A. preparing an ex ovo CAM;
• and
• B. adding a cell-infused media to the CAM, to form an ex ovo tumor xenograft,
• wherein the cell-infused media comprises tumor cells, optionally wherein the tumor cells are suspended in a media;
• or a gel;
• or a hydrogel and a combination thereof, to form the cell-infused media, and
• optionally further comprising the step of creating a superficial injury to the CAM and placing the cell-infused media in contact with the superficial injury;
• and
• optionally comprising an experimental incubation wherein the CAM is incubated for from 0 hours to about 144 hours after adding the cell-infused media to the CAM.

A method for testing a therapeutic compound via an ex ovo quail egg xenograft assay comprising the steps of:

• A. transferring a quail embryo to a well in a standardized multi-well cell culture plate;
• B. subjecting the embryo to a CAM formation incubation of from about 60 hours to about 144 hours to develop a CAM, wherein the CAM comprises a CAM surface;
• C. culturing at least one cell to form a plurality of cells;
• D. suspending the plurality of cells in a media, optionally a gel or a hydrogel, to form a cell-infused media;
• E. adding the cell-infused media to a scaffold;
• F. creating a superficial injury on the CAM surface;
• G. placing a ring on the CAM surface, wherein the ring at least partially encloses the superficial injury;
• H. placing the scaffold on the CAM surface in contact with the superficial injury, wherein the scaffold is placed within the ring;
• I. combining a delivery media with a therapeutic compound;
• J. placing the delivery media in the ring and on top of the scaffold;
• K. subjecting the CAM to an experimental incubation;
• or wherein the experimental incubation is from about 0 hours to about 144 hours;
• L. administering a marker to a marker location selected from the well, the embryo, the CAM and a combination thereof;
• optionally wherein the marker is a luminescent marker, a radioactive marker, a fluorescent marker, and a combination thereof;
• a luminescent marker, a fluorescent marker;
• and a combination thereof;
• or a bioluminescent marker;
• or a fluorescent marker;
• and
• M. detecting the marker to generate experimental data.