Hyaluronic acid derivative having a reduced polarity, method of preparation thereof, composition and use thereof

Abstract

The invention relates to a method of preparation of hyaluronic acid derivative, that has some carboxyl groups (—COOH) replaced by the primary hydroxyl group (—CH2—OH). The method of preparation is based on the carboxyl group activation using 4-(4,6-dimethoxy-1,3,5-triazine-2-yl)-4-methylmorpholinium chloride agent and subsequent reduction with NaBH4. The hyaluronic acid derivatives prepared according to invention are biocompatible and biodegradable, showing increased resistance to thermal and enzymatic degradation. Formula for annotation (I): I═R1-(A)X-(B)Y—R2 wherein A is a structural fragment of a linear chain: B is a structural fragment of a linear chain: R1 is the structural fragment of the end of the linear chain: R2 is the structural fragment of the end of the linear chain: M can be hydrogen or any alkali metal cation, X is an index indicating the number of structural fragments A within the chain, Y is an index indicating the number of structural fragments B within the chain, while at the same time: structural fragments A and B are randomly distributed in the linear chain, and the sum of the X+Y indexes is in the range of 500 to 2,500, and the ratio of the X/Y indexes is in the range of 3/1 to 1/4.

Dependencies are inferred from the claim text.