Viral vectors and use thereof in adoptive cellular therapy
AssigneeImmatics US, Inc.
InventorsMelinda MATA, Yannick BULLIARD, Mamta KALRA
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Abstract
A vector containing a first nucleotide sequence S1 encoding a protein Z1, a second nucleotide sequence S2 encoding a protein Z2, a third nucleotide sequence S3 encoding a protein Y1, and a fourth nucleotide sequence S4 encoding a protein Y2, in which Z1 and Z2 form a first dimer and Y1 and Y2 form a second dimer, in which the first dimer Z1Z2 is different from the second dimer Y1Y2.
Claims20
A T cell transduced with a vector comprising a nucleotide sequence S1 encoding a CD8α polypeptide, a nucleotide sequence S2 encoding a CD8β polypeptide, a nucleotide sequence S3 encoding a T cell receptor (TCR)α polypeptide, and a nucleotide sequence S4 encoding a TCRβ polypeptide, wherein the nucleotide sequences are arranged in tandem in a 5′ to 3′ orientation of S2-S1-S4-S3.
A method of preparing a T cell for immunotherapy, comprising
- activating the T cell in the presence of a statin,
- transducing the activated T cell with a vector comprising a nucleotide sequence S1 encoding a CD8α polypeptide, a nucleotide sequence S2 encoding a CD8β polypeptide, a nucleotide sequence S3 encoding a T cell receptor (TCR) a polypeptide, and a nucleotide sequence S4 encoding a TCRβ polypeptide,
- wherein the nucleotide sequences are arranged in tandem in a 5′ to 3′ orientation of S2-S1-S4-S3, and
- expanding the transduced T cell.
Dependencies are inferred from the claim text.