Method for regulating in vitro biosynthesis activity by knocking-out of nuclease system
AssigneeKangma-Helthcode (Shanghai) Biotech Co., Ltd
InventorsMin GUO, Lingxuan JIANG, Zhuxia ZHENG, Xue YU
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Abstract
What provided is a method for regulating in vitro biosynthesis activity by knocking-out of a nuclease system, comprising: screening five nucleases among numerous nucleases, and performing down-regulation or knocking-out on one of the five nucleases (e.g., EXN53). The method can improve the stability of nucleic acid and the protein production efficiency of an in vitro protein synthesis system.
Claims16
A yeast cell extract for in vitro protein synthesis, wherein, the yeast cell extract comprises EXN53 protein, and content of the EXN53 protein in the yeast cell extract is equal to or less than 10%.
Dependencies are inferred from the claim text.