Improved production of secreted proteins in yeast cells
AssigneeEuropean Molecular Biology Laboratory
InventorsThomas Desfougeres, Thierry Dulermo, Georges Pignede, Lars Steinmetz, Andreas S. Johansson
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Abstract
The present invention relates to a yeast cell producing at least one secreted protein of interest, wherein said cell comprises at least one additional fungal gene showing increased expression and/or overexpression, showing reduced expression and/or inactivation, wherein said gene improves the production of the at least one secreted protein of interest. The present invention further relates to respective methods for production and uses of the yeast cell.
Claims18
A cell of Saccharomyces cerevisiae, producing at least one secreted protein of interest, wherein at least one of:
- the cell comprises at least one fungal gene selected from the group consisting of ENO2, NMA2, PRY2, SUT074, TFG2, AVT2, TRM10, BNA7, and TOM22, wherein said at least one fungal gene shows at least one of increased expression and overexpression;
- and
- the cell comprises at least one further fungal gene selected from the group consisting of TLG2, MNT2, TPO2, ATG33, THR4, INP51, CUT901, YDR262W, MRP10, NDC1, and CMC1, wherein the at least one further fungal gene shows at least one of reduced expression and inactivation.
A method for producing a yeast cell producing at least one secreted protein of interest, comprising introducing into the cell producing at least one secreted protein of interest at least one fungal gene selected from the group consisting of ENO2, NMA2, PRY2, SUT074, TFG2, AVT2, TRM10, BNA7, and TOM22, wherein the at least one fungal gene shows at least one of increased expression and overexpression, and/or wherein the cell comprises at least one further fungal gene selected from the group consisting of TLG2, MNT2, TPO2, ATG33, THR4, INP51, CUT901, YDR262W, MRP10, NDC1, and CMC1, wherein the at least one further fungal gene shows at least one of reduced expression inactivation.
(canceled)
Dependencies are inferred from the claim text.