Whooping cough antigen recombinant expression vector, and engineered strain and use thereof

Abstract

Provided are a recombinant expression vector for whooping cough antigens, an engineered bacterium and use thereof. The recombinant expression vector for whooping cough antigens comprises a protein expression cassette for pertactin, a gene for resistance screening, and an upstream recombinant nucleic acid fragment and a downstream recombinant nucleic acid fragment of filamentous haemagglutinin gene, wherein the protein expression cassette for pertactin is located between the upstream recombinant nucleic acid fragment and the downstream recombinant nucleic acid fragment of the filamentous haemagglutinin gene, and the upstream recombinant nucleic acid fragment and the downstream recombinant nucleic acid fragment of the filamentous haemagglutinin gene can undergo homologous recombination with the upstream and downstream of the filamentous haemagglutinin gene, respectively. The gene of FHA protein in the recombinant expression vector is knocked out and the copies PRN protein gene are increased, so that the Bordetella pertussis genetically engineered strain can efficiently overexpress PRN protein antigen and PT protein, and the expressed PRN protein antigen and PT protein exist in the cells and the supernatant respectively, thereby facilitating subsequent separation and purification of the antigens.

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