Treating & preventing e coli infections
AssigneeSNIPR Biome ApS
InventorsJakob Krause HAABER, Szabolcs SEMSEY, Mette GROVE, Birgitte DAMHOLT, Dziuginta JASINSKYTE, Yilmaz Emre GENÇAY
Life of this patent
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Abstract
The invention relates to methods and compositions for treating or preventing an infection by E coli cells in human or animal subjects. The method comprises administering to the subject a plurality of transduction particles that encode a nuclease for targeting the genomes of B2 phylogroup E coli cells.
Claims31
A composition comprising a plurality of transduction particles, wherein (a) each transduction particle comprises a nucleic acid encoding a Cas nuclease and a nucleic acid encoding a crRNA or guide RNA that is operable with the Cas nuclease for targeting the genomes of Escherichia coli (E. coli) cells,
- wherein (i) each transduction particle is a phage and the nucleic acid encoding the Cas nuclease is integrated into the genome of the phage, or (ii) each transduction particle is a phage capsid and the nucleic acid encoding the Cas nuclease is a phagemid contained in the phage capsid,
- (b) the E. coli cells are cells of E. coli phylogroup B2;
- and
- (c) wherein the plurality of transduction particles comprises a first type of transduction particle and a second type of transduction particle, wherein the first type of transduction particle comprises a first adhesion moiety that is capable of recognizing and binding to LPS displayed on the surface of phylogroup B2 E. coli cells, and the second type of transduction particle comprises a second adhesion moiety that is capable of recognizing and binding to Tsx displayed on the surface of phylogroup B2 E. coli cells.
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A composition comprising a plurality of transduction particles, wherein
- (a) each transduction particle comprises a nucleotide sequence (N1) encoding a Cas nuclease for targeting the genomes of E. coli cells and a nucleic acid encoding a crRNA or guide RNA that is operable with the Cas nuclease for targeting the genomes of E. coli cells,
- (b) the E. coli cells are cells of E. coli phylogroup B2;
- and
- each transduction particle comprises an adhesion moiety that is capable of recognizing and binding to a cognate moiety selected from the group consisting of a LPS, LamB and Tsx displayed on the surface of phylogroup B2 E. coli cells,
- wherein each transduction particle is a synthetic T-even phage comprising an insertion of N1 into the genome of the phage, wherein the insertion is between the pin (protease inhibitor) gene and the iPII (internal protein) gene.
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Dependencies are inferred from the claim text.