A method for inducing ipsc differentiation to obtain cd34+cells and nk cells and application thereof

A culture medium for inducing iPSC differentiation to obtain CD34+cells, characterized in that the culture medium comprises a first stage medium, a second stage medium, a third stage medium, and a fourth stage medium;

• the first stage culture medium is an E8 complete culture medium containing a ROCK pathway inhibitor and polyvinyl alcohol;
• the second stage culture medium is an E8 complete culture medium containing a GSK-3B inhibitor;
• the third stage culture medium comprises a SPM1 culture medium and a SPM2 culture medium;
• the fourth stage culture medium is a SPM3 culture medium;
• the SPM1 culture medium comprises stepro-34 complete culture medium, DMEM/F12 culture medium, L-glutamine, ascorbic acid, ITS-X, BMP4, VEGF, bFGF;
• the SPM2 culture medium comprises the SPM1 culture medium and an inhibitor of TGF-β type I receptors ALK5, ALK4 and ALK7;
• the SPM3 culture medium comprises stepro-34 complete culture medium, DMEM/F12 culture medium, L-glutamine, ascorbic acid, ITS-X, bFGF, VEGF, SCF, TPO, FLT-3L.