Silk-based articles having variable active agent release rates
OwnerDavid L. Kaplan, Junqi Wu +1inventors
InventorsDavid L. Kaplan, Junqi Wu, Vincent P. Fitzpatrick
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Abstract
Coated silk fibroin articles having variable active agent release rates and methods of making and using the same are disclosed. The coating can have variable water permeability. In some cases, the variable permeability is temperature-dependent. In these cases, the release rate of the active agent can be controlled by adjusting the water permeability by raising and lowering the temperature, so long as the temperature is maintained below a melting point of the coating. Elevating the temperature above the melting point causes loss of a portion of the coating, thereby permanently increasing the release rate.
Claims24
-5. (canceled)
A coated silk fibroin article having controllable active agent release rates, the coated silk fibroin article comprising:
- a solid silk fibroin dosage form core comprising an active agent;
- and
- a polycaprolactone coating encapsulating the solid silk fibroin dosage form core,
- wherein a core-only comparison dosage form comprising the solid silk fibroin dosage form core comprising the active agent and lacking any coating has a core-only active agent release rate,
- wherein the coated silk fibroin article has a first active agent release rate when the polycaprolactone coating is above a first temperature threshold and below a second temperature threshold, wherein the second temperature threshold is a melting temperature of the polycaprolactone coating, wherein the first temperature threshold is a transition temperature below which the polycaprolactone coating is substantially water impermeable and above which the polycaprolactone coating is at least partly water permeable,
- wherein the coated silk fibroin article has a second active agent release rate when the polycaprolactone coating is below the first temperature threshold, wherein the second active agent release rate is less than the first active agent release rate, and
- wherein the coated silk fibroin article loses at least part of the polycaprolactone coating when the polycaprolactone coating is above the second temperature threshold and adopts a third active agent release rate between the first active agent release rate and the core-only active agent release rate, wherein the third active agent release rate increases as more of the polycaprolactone coating is lost from the coated silk fibroin article.
The coated article of claim 67, wherein the solid dosage form core is a silk fibroin foam.
The coated article of claim 67, wherein the active agent has a molecular weight of 10 kg/mol or less, 5 kg/mol or less, 1 kg/mol or less, or 750 g/mol or less.
The coated article of claim 67, wherein the polycaprolactone coating includes a polycaprolactone having a number average molecular weight of at least 5 kDa, at least 10 kDa, at least 20 kDa, at least 40 kDa, at least 50 kDa, at least 60 kDa, or at least 70 kDa and at most 500 kDa, at most 250 kDa, or at most 150 kDa, wherein the number average molecular weight is optionally at least 20 kDa, wherein the number average molecular weight is optionally at least 40 kDa, wherein the number average molecular weight is optionally at least 50 kDa.
The coated article of claim 67, wherein the first temperature threshold is higher than normal human body temperature.
The coated article of claim 67, wherein the first temperature threshold is at least 20° C., at least 25° C., at least 30° C., at least 35° C., or at least 40° C.
The coated article of claim 67, wherein the second temperature threshold is a melting point of pure polycaprolactone.
-21. (canceled)
The coated article of claim 67, the polycaprolactone coating having a substantially uniform thickness.
The coated article of claim 67, the polycaprolactone coating having an average thickness of between 1 m and 5 mm.
The coated article of claim 67, wherein the polycaprolactone coating accounts for no more than 50% by weight of the coated article and at least 1% by weight of the coated article.
The coated article of claim 67, the solid dosage form core and/or the polycaprolactone coating comprising an acid-activated protease.
The coated article of claim 67, wherein the coated article comprises a flexural strength of at least 50 MPa.
The coated article of claim 67, the coated article and/or the solid dosage form core having a density of at least 1.20 g/cm3.
-62. (canceled)
A method of delivering an active agent to a target location with a controlled release rate, the method comprising:
- a) administering a polycaprolactone-coated dosage form to a target location, the polycaprolactone-coated dosage form having a solid dosage form core comprising an active agent and a polycaprolactone coating encapsulating the solid dosage form core;
- and
- b) subsequent to step a), elevating temperature of the polycaprolactone-coated dosage form to above a first temperature threshold and below a second temperature threshold, wherein the second temperature threshold is a melting temperature of the polycaprolactone coating, wherein the first temperature threshold is a transition temperature below which the polycaprolactone coating is substantially water impermeable and above which the polycaprolactone coating is at least partly water permeable, thereby allowing water to pass between the target location and the solid dosage form core and providing a first active agent release rate of the active agent from the polycaprolactone-coated dosage form to the target location.
A coated article having controllable active agent release rates, the coated article comprising:
- a solid dosage form core comprising an active agent;
- and
- a polycaprolactone coating encapsulating the solid dosage form core,
- wherein a core-only comparison dosage form comprising the solid dosage form core comprising the active agent and lacking any coating has a core-only active agent release rate,
- wherein the coated article has a first active agent release rate when the polycaprolactone coating is above a first temperature threshold and below a second temperature threshold, wherein the second temperature threshold is a melting temperature of the polycaprolactone coating, wherein the first temperature threshold is a transition temperature below which the polycaprolactone coating is substantially water impermeable and above which the polycaprolactone coating is at least partly water permeable,
- wherein the coated article has a second active agent release rate when the polycaprolactone coating is below the first temperature threshold, and
- wherein the coated article loses at least part of the polycaprolactone coating when the polycaprolactone coating is above the second temperature threshold and adopts a third active agent release rate between the first active agent release rate and the core-only active agent release rate, wherein the third active agent release rate increases as more of the polycaprolactone coating is lost from the coated article.
(canceled)
Dependencies are inferred from the claim text.