Screening methods using gprc6a taste receptors and pet food products and compositions prepared using the same
AssigneeMars, Incorporated
InventorsScott Joseph McGrane, Matthew Ronald Gibbs, Boris Klebansky, Richard Masten Fine
Life of this patent
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Abstract
The presently disclosed subject matter relates to methods of screening raw materials and pet food products to manufacture a palatable pet food product. The presently disclosed subject matter also relates to methods for identifying compounds that modulate the activity and/or expression of a taste receptor.
Claims20
A method for identifying a compound that modulates the activity of a canine GPRC6A receptor comprising the amino acid sequence set forth in SEQ ID NO:
- 5, the method comprising
- (a) detecting an in silico interaction between a test agent and one or more amino acid residues in a seven transmembrane domain (7TM) or a Venus flytrap domain (VFT) of the receptor,
- (b) contacting the test agent with a cell comprising an exogenous feline GPRC6A receptor or a functional fragment thereof,
- (c) measuring a biological activity of the exogenous feline GPRC6A receptor, and
- (d) selecting as the compound, a test agent that increases or decreases the activity of the polypeptide.
A method for identifying a compound that modulates the activity of a canine GPRC6A receptor comprising the amino acid sequence set forth in SEQ ID NO:
- 5, the method comprising
- (a) detecting an in silico interaction between a test agent and one or more amino acid residues in a seven transmembrane domain (7TM) and a Venus flytrap domain (VFT) of the receptor,
- (b) contacting the test agent with a cell comprising an exogenous feline GPRC6A receptor or a functional fragment thereof,
- (c) measuring a biological activity of the exogenous feline GPRC6A receptor, and
- (d) selecting as the compound, a test agent that increases or decreases the activity of the polypeptide.
Dependencies are inferred from the claim text.