Inot1824 transgenic maize

A transgenic maize plant cell comprising a nucleotide sequence comprising a Zein promoter, an amy797E coding region which is operably linked to said promoter, a CaMV 35S terminator element which is operably linked to said amy797E coding region, and a nopaline synthase terminator element, wherein said nucleotide sequence does not contain a ZmUbiInt promoter and an operably linked phosphomannose isomerase coding region between said terminator elements optionally wherein:

• (i) the ZmUbiInt promoter, the amy797E coding region which is operably linked to said promoter, the CaMV 35S terminator element which is operably linked to said amy797E coding region are located in the maize plant cell genomic location of the SYN-E3272-5 transgenic locus;
• (ii) wherein a selectable marker or scoreable is absent from said maize plant cell genomic location, and/or (iii) wherein the nopaline synthase terminator element is not separated from the CaMV 35S terminator element by DNA encoding a selectable marker protein, a scoreable marker protein, or a protein conferring a useful trait.

A transgenic maize plant cell comprising a nucleotide sequence comprising a ZmUbiInt promoter, an amy797E coding region which is operably linked to said promoter, a CaMV 35S terminator element which is operably linked to said amy797E coding region, and a nopaline synthase terminator element, wherein said nucleotide sequence does not contain a phosphomannose isomerase coding region between said terminator elements, optionally wherein:

• (i) the ZmUbiInt promoter, the amy797E coding region which is operably linked to said promoter, the CaMV 35S terminator element which is operably linked to said amy797E coding region are located in the maize plant cell genomic location of the SYN-E3272-5 transgenic locus;
• (ii) wherein a selectable marker or scoreable is absent from said maize plant cell genomic location, and/or (iii) wherein the nopaline synthase terminator element is not separated from the CaMV 35S terminator element by DNA encoding a selectable marker protein, a scoreable marker protein, or a protein conferring a useful trait.

A transgenic maize plant cell comprising a nucleotide sequence comprising a Zein promoter, an amy797E coding region which is operably linked to said promoter, a CaMV 35S terminator element which is operably linked to said amy797E coding region, and a nopaline synthase terminator element, wherein said nucleotide sequence does not contain a ZmUbiInt promoter and an operably linked phosphomannose isomerase coding region, optionally wherein:

• (i) the ZmUbiInt promoter, the amy797E coding region which is operably linked to said promoter, the CaMV 35S terminator element which is operably linked to said amy797E coding region are located in the maize plant cell genomic location of the SYN-E3272-5 transgenic locus;
• (ii) wherein a selectable marker or scoreable is absent from said maize plant cell genomic location, and/or (iii) wherein the nopaline synthase terminator element is not separated from the CaMV 35S terminator element by DNA encoding a selectable marker protein, a scoreable marker protein, or a protein conferring a useful trait.

A transgenic maize plant cell comprising a nucleotide sequence comprising a ZmUbiInt promoter, an amy797E coding region which is operably linked to said promoter, a CaMV 35S terminator element which is operably linked to said amy797E coding region, and a nopaline synthase terminator element, wherein said nucleotide sequence does not contain a phosphomannose isomerase coding region, optionally wherein:

• (i) the ZmUbiInt promoter, the amy797E coding region which is operably linked to said promoter, the CaMV 35S terminator element which is operably linked to said amy797E coding region are located in the maize plant cell genomic location of the SYN-E3272-5 transgenic locus;
• (ii) wherein a selectable marker or scoreable is absent from said maize plant cell genomic location, and/or (iii) wherein the nopaline synthase terminator element is not separated from the CaMV 35S terminator element by DNA encoding a selectable marker protein, a scoreable marker protein, or a protein conferring a useful trait.

A transgenic maize plant cell comprising an INOT1824 transgenic locus comprising the Zein promoter, the amy797E coding region which is operably linked to said promoter, the CaMV 35S terminator element which is operably linked to said amy797E coding region, and the nopaline synthase terminator element of a SYN-E3272-5 transgenic locus, allelic variants thereof, or other variants thereof, wherein DNA of said original SYN-E3272-5 transgenic locus, allelic variants thereof, or other variants thereof comprising a ZmUbiInt promoter and an operably linked phosphomannose isomerase coding region is absent.

A transgenic maize plant cell comprising an INOT1824 transgenic locus comprising an insertion and/or substitution of a DNA element comprising a cognate guide RNA recognition site (CgRRS) in a DNA junction polynucleotide in the SYN-E3272-5 transgenic locus comprising SEQ ID NO:

• 1, in DNA junction polynucleotide of the SYN-E3272-5 locus in the deposited seed of accession No. PTA-9972, or an allelic variant thereof.

A DNA molecule comprising SEQ ID NO:

• 8, 9, 10, 11, 12, or 16.

A method of modifying a transgenic maize plant cell comprising:

• obtaining a SYN-E3272-5 maize event plant cell, a representative sample of which was deposited at the ATCC under accession No. PTA-9972, comprising a nucleotide sequence comprising a Zein promoter, an amy797E coding region which is operably linked to said promoter, a CaMV 35S terminator element which is operably linked to said amy797E coding region, a ZmUbiInt promoter and an operably linked phosphomannose isomerase coding region, and a nopaline synthase terminator element;
• and modifying said nucleotide sequence to eliminate functionality of said phosphomannose isomerase coding region and/or to substantially, essentially, or completely remove said phosphomannose isomerase coding region, and optionally to eliminate functionality of, or substantially, essentially, or completely remove, said ZmUbiInt promoter.

A method of modifying a transgenic maize plant cell comprising:

• obtaining a SYN-E3272-5 maize event plant cell, a representative sample of which was deposited at the ATCC under accession No. PTA-9972, comprising a nucleotide sequence comprising a Zein promoter, an amy797E coding region which is operably linked to said promoter, a CaMV 35S terminator element which is operably linked to said amy797E coding region, a ZmUbiInt promoter and an operably linked phosphomannose isomerase coding region, and a nopaline synthase terminator element;
• and modifying said nucleotide sequence to substantially, essentially, or completely remove said phosphomannose isomerase coding region, and optionally substantially, essentially, or completely remove said ZmUniInt promoter.