Diagnostic method for multiple sclerosis
AssigneeDeutsches Krebsforschungszentrum Stiftung des Offentlichen Rechts
InventorsHarald ZUR HAUSEN, Timo BUND, Ethel-Michele DE VILLIERS-ZUR HAUSEN, Isolde SCHUSTER, Hans GRÖNLUND
Life of this patent
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Abstract
The present invention provides a method for diagnosing and monitoring MS on the basis of an immunoassay using MSBI1.176 or MSBI2.176 Rep protein or fragments thereof as an antigen for binding antibodies against MSBI1.176 or MSBI2.176 Rep protein from a subject's sample.
Claims15
An immunoassay for the detection and/or measuring of an antibody against MSBI Rep protein in a subject's sample, wherein the method comprises:
- (a) coating a solid support with a capture antigen being mutant MSBI Rep oligomer via a specific binding pair;
- (b) contacting the capture antigen with the sample under conditions to form a capture antigen:
- anti-MSBI Rep antibody complex;
- (c) removing unbound sample;
- (d) contacting the capture antigen:
- anti-MSBI Rep antibody complex with a tracer compound being wild-type MSBI Rep oligomer conjugated to a detectable label or an anti-human antibody conjugated to a detectable label to form a capture antigen:
- anti-MSBI Rep antibody:
- tracer compound complex;
- (e) removing unbound tracer compound;
- (f) adding a dye, chemiluminescent or fluorescent substrate;
- and
- (g) detecting and/or measuring the capture antigen:
- anti-MSBI Rep antibody:
- tracer compound complex via a detectable reaction of the substrate.
An immunoassay for the detection and/or measuring of an antibody against MSBI Rep protein in a subject's sample, wherein the method comprises:
- (a) coating a solid support with a capture antigen being wild-type MSBI Rep aggregate via a specific binding pair;
- (b) contacting the capture antigen with the sample under conditions to form a capture antigen:
- anti-MSBI Rep antibody complex;
- (c) removing unbound sample;
- (d) contacting the capture antigen:
- anti-MSBI Rep antibody complex with a tracer compound being wild-type MSBI Rep aggregate conjugated to a detectable label or an anti-human antibody conjugated to a detectable label to form a capture antigen:
- anti-MSBI Rep antibody:
- tracer compound complex;
- (e) removing unbound tracer compound;
- (f) adding a dye, chemiluminescent or fluorescent substrate
- (g) detecting and/or measuring the capture antigen:
- anti-MSBI Rep antibody:
- tracer compound complex via a detectable reaction of the substrate.
A kit, comprising (a) MSBI Rep protein or fragment thereof as a capture antigen that is immobilized via a specific binding pair to a solid phase, (b) MSBI Rep protein or fragment thereof that is conjugated to a detectable label or an anti-human antibody that is conjugated to a detectable label as a tracer compound, (c) a dye, chemiluminescent or fluorescent substrate and (d) instructions for using the kit in a method for diagnosing or monitoring Multiple Sclerosis.
Dependencies are inferred from the claim text.