Methods and systems for three-dimensional imaging of a transparent biological object in a biological sample by full-field optical tomography

A method for three-dimensional imaging of a transparent biological object in a biological sample by full-field optical tomography, the three-dimensional imaging method comprising:

• positioning the sample in the vicinity of an object focal plane of a microscope lens, said microscope lens comprising a given optical axis (Δ);
• illuminating the sample in transmission by an illumination beam of spatially incoherent light with a given central wavelength (λ);
• relatively displacing said microscope lens relative to said sample, along an axial direction parallel to the optical axis of the microscope lens, to define a plurality of positions of the sample, each position corresponding to a section of said biological object centred on the object focal plane of the microscope lens;
• and for each position of the sample, producing at least one first image of an object field of said section comprising:
• acquiring, by a two-dimensional acquisition device comprising a plurality of elementary detectors arranged in a detection plane, a plurality of two-dimensional interferometric signals resulting from optical interference between the illumination beam incident on the object field and a beam scattered by said object field, wherein said detection plane is optically conjugated with the object focal plane of the microscope lens by an imaging optical system comprising said microscope lens;
• calculating, by a processing unit, said at least one first image, from said plurality of two-dimensional interferometric signals.

A three-dimensional imaging system for imaging a transparent biological object in a biological sample by full-field optical tomography, the imaging system comprising:

• a light source configured for the emission of an illumination beam of spatially incoherent light, of given central length, said illumination beam being configured to illuminate the sample in transmission;
• an optical imaging system comprising a microscope lens with a given optical axis (A) and a given object focal plane in the vicinity of which, in operation, the sample is positioned;
• means for relatively displacing said microscope lens relative to said sample, along an axial direction parallel to the optical axis of the microscope lens;
• a two-dimensional acquisition device comprising a plurality of elementary detectors arranged in a detection plane, said detection plane being optically conjugated with the object focal plane of the microscope lens by said optical imaging system;
• and
• a processing unit;
• and wherein, for each section of a plurality of sections of said biological object:
• said three-dimensional imaging system is configured for the acquisition, by said two-dimensional acquisition device, of a plurality of two-dimensional interferometric signals resulting from optical interference between said illumination beam and a beam scattered by an object field of said section;
• said processing unit is configured to calculate from said plurality of two-dimensional interferometric signals at least one first image of said object field of said section.