Spatially Mapped RNA Sequencing from Single Cells

Abstract

Provided herein are methods of making a spatially-barcoded microarray, the method comprising the steps of: providing distinguishable mini-barcoded beads, wherein the distinguishable mini-barcoded beads comprise a plurality of distinct bead populations, with each bead member of a distinct population having an identical mini-barcode sequence; simultaneously delivering the plurality of distinguishable barcoded beads to a plurality of wells of a microarray, wherein a single bead is provided to each well; imaging the plurality of wells to identify the population type of each bead in each of the plurality of wells and thereby identify the mini-barcode in each well; removing the mini-barcodes from the beads and connecting the mini-barcode or a polymerase product of the mini-barcode to a surface of the well in which the bead is located; and removing the beads from the wells.

Dependencies are inferred from the claim text.