Biocatalysts and methods for the synthesis of pregabalin intermediates
AssigneeEnzymaster (Ningbo) Bio-Engineering Co., Ltd
InventorsYingxin ZHANG, Haibin CHEN, Marco BOCOLA, Baoqin CAI, Zhaoqi ZHANG, Xiao LUO, Yaoyao JI, Chengxiao ZHANG, Ruimei HONG
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Abstract
Provided is an engineered polypeptide capable of catalyzing the asymmetric hydrolysis of 3-isobutylglutarimide to generate (R)-(−)-3-(carbamoylmethyl)-5-methylhexanoic acid. The engineered polypeptide has high stereoselectivity, high catalytic activity, good process stability and thermal stability, and tolerance to high product concentrations, and has good prospects for industrial applications.
Claims20
An engineered hydantoinase polypeptide that catalyzes the asymmetric hydrolysis of 3-isobutylglutarimide to generate (R)-(−)-3-(carbamoylmethyl)-5-methylhexanoic acid with an entantiomeric excess (ee) value of at least 97%, wherein said polypeptide comprises an X64 substitution and a at least 90% sequence identity to reference sequence SEQ ID NO.2, wherein the amino acid residue at residue position X64 is selected from the group consisting of I, T, S and A.
Dependencies are inferred from the claim text.