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AssigneeBeijing Dabeinong Biotechnology Co., Ltd.
InventorsXiang XIAO, Qingfang SONG, Qing TAO, Caihong YU
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The present invention relates to a mutated hydroxyphenylpyruvate dioxygenase (HPPD) polypeptide, and a coding gene and use thereof. The mutated HPPD polypeptide retains the activity of catalyzing the conversion of 4-hydroxyphenylpyruvic acid into homogentisic acid or homogentisate, and has lower sensitive to an HPPD inhibitor herbicide than that to wild type HPPD. An amino acid sequence corresponding to the amino acid sequence as shown in SEQ ID NO: 1 comprises amino acid mutation at the following sites: an F372 site is substituted by A, G or V, and an F383 site is substituted by W. The present invention discloses for the first time that the combination mutation at the 372 site and the 383 site of HPPD polypeptides from different species sources can endow plants with synergistic tolerance to HPPD inhibitor herbicides, and the application prospect in plants is wide.
A mutant hydroxyphenylpyruvate dioxygenase polypeptide, which retains the activity of catalyzing the conversion of 4-hydroxyphenylpyruvic acid into homogentisic acid or homogentisate and is less sensitive to an HPPD-inhibitor herbicide than the wild-type HPPD, characterized by comprising the following amino acid mutations at the positions corresponding to the positions of the amino acid sequence as set forth in SEQ ID NO:
Dependencies are inferred from the claim text.