Transmissible elements comprising pathway modification systems and exogenous nucleic acids for the production of molecules of interest

A method for increasing production of a first molecule of interest (MOI) in a recipient bacterium, comprising introducing a transmissible element into the recipient bacterium, wherein the transmissible element comprises:

• A) at least one nucleic acid that is exogenous to the recipient bacterium for the production of a first molecule of interest (MOI) in the recipient bacterium;
• and
• B) a Pathway Modulation System (PMS) which encodes a nucleic acid modifier to modulate a target endogenous nucleic acid or protein for the production, consumption, or degradation of the first MOI in the recipient bacterium, wherein the nucleic acid modifier comprises:
• i) a base editor;
• ii) a prime editor;
• iii) an RNAi system;
• iv) a CRISPRi system;
• or
• v) a nuclease,
• wherein the transmissible element comprises a conjugative plasmid, phage or phagemid.