Recombinant microorganism used for producing cdp-choline, and method for producing cdp-choline using said recombinant microorganism
AssigneeKyowa Hakko Bio Co., Ltd.
InventorsKazumasa HORI, Toshihiko MORITA, Tsuneyo NISHINO, Tetsuro UJIHARA
Life of this patent
Estimating the term…
Abstract
Provided are a recombinant microorganism having an ability to produce heterologous CTP:phosphocholine cytidylyltransferase, in which the CTP:phosphocholine cytidylyltransferase is a polypeptide having a CTP:phosphocholine cytidylyltransferase activity and consisting of a specific amino acid sequence, and a method for producing CDP-choline using the recombinant microorganism.
Claims5
A recombinant microorganism having an ability to produce heterologous CTP:phosphocholine cytidylyltransferase, wherein the CTP:phosphocholine cytidylyltransferase is any one polypeptide selected from the following (A1) to (A3) and (B1) to (B3):
- (A1) a polypeptide consisting of the amino acid sequence represented by SEQ ID NO:
- 10;
- (A2) a polypeptide having a CTP:phosphocholine cytidylyltransferase activity and consisting of an amino acid sequence having an identity of at least 60% or more with the amino acid sequence represented by SEQ ID NO:
- 10;
- (A3) a polypeptide having a CTP:phosphocholine cytidylyltransferase activity and consisting of an amino acid sequence in which 1 to 20 amino acids are deleted, substituted, inserted, or added in the amino acid sequence represented by SEQ ID NO:
- 10;
- (B1) a polypeptide consisting of the amino acid sequence represented by SEQ ID NO:
- 14;
- (B2) a polypeptide having a CTP:phosphocholine cytidylyltransferase activity and consisting of an amino acid sequence having an identity of at least 60% or more with the amino acid sequence represented by SEQ ID NO:
- 14;
- and
- (B3) a polypeptide having a CTP:phosphocholine cytidylyltransferase activity and consisting of an amino acid sequence in which 1 to 20 amino acids are deleted, substituted, inserted, or added in the amino acid sequence represented by SEQ ID NO:
- 14.
Dependencies are inferred from the claim text.