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US 2025/0042938 A1Published application
Method for the purification of recombinant proteins using oil body proteins
AssigneeCore Biogenesis
InventorsChouaïb MEZIADI, Daniel-Isui AGUILAR-SALVADOR, Michel NEY
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Abstract
The invention relates to a method for the purification of recombinant proteins. Particularly, the present invention relates to a method for the purification of recombinant proteins produced by a first genetically engineered organism by mixing the first genetically engineered organism or part thereof with oil body proteins which are not obtained from the first genetically engineered organism.
Claims15
A method for the purification of recombinant proteins produced by a first genetically engineered organism, the method comprising the following steps;
- Providing the first genetically engineered organism expressing the recombinant proteins or a part thereof;
- Mixing the first genetically engineered organism or part thereof with oil body proteins which are not obtained from the first engineered organism, wherein the recombinant proteins and/or the oil body proteins are fused with an affinity ligand which allows forming complexes comprising the oil body proteins and the recombinant proteins;
- Recovering the complexes comprising the oil body proteins and the recombinant proteins by flotation of the mix.
Dependencies are inferred from the claim text.