Method for glycosylation modification of proteins and/or polypeptides

A method for glycosylation modification of proteins and/or polypeptides, characterized in that the method comprises the following steps:

• the starting materials including glycosyl sulfinates, proteins and/or polypeptides, and oxidants are allowed to react in a solvent, to obtain glycosylation-modified proteins and/or polypeptides;
• wherein, the proteins and/or polypeptides contain sulfhydryl and/or disulfide bonds;
• the structure of the glycosyl sulfinate is as represented by formula W:
• wherein, n is selected from 0 or 1;
• a is selected from 3 or 4;
• R is each independently selected from L1Rx;
• or two adjacent R are linked to form a ring, while the other R is each independently selected from L1Rx. The ring is either unsubstituted or substituted with one or more L1Rx;
• L1 is selected from absence or C1-2 alkylene, and Rx is selected from the group consisting of H, OH, C1-6 alkyl, OAc (acetyloxy), OBn (benzyloxy), OR8, NR9R10,
• i is an integer selected from 0 to 6;
• R11, R12, R13, and R14 are each independently selected from L2Ry;
• L2 is selected from absence or C1-2 alkylene, and Ry is selected from the group consisting of H, OH, C1-6 alkyl, OAc, OBn, OR8, and NR9R10;
• R8 is selected from C1-6 alkyl;
• R9 is selected from the group consisting of H, C1-6 alkyl, Ac (acetyl), and Bn (benzyl);
• R10 is selected from the group consisting of H, C1-6 alkyl, Ac, and Bn;
• j is selected from 1, 2, or 3;
• Mj+ is a j-valent cation.