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US 2025/0040524 A1Published application
Methods for generating genetically modified animals
AssigneeeGenesis, Inc.
InventorLuhan YANG
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AssigneeeGenesis, Inc.
InventorLuhan YANG
Estimating the term…
The present disclosure provides methods of generating multiplexed genetically modified animals, for example, porcine endogenous retrovirus (PERV)-inactivated pigs. The disclosure also provides methods of improving the birth rate of multiplexed genetically modified animals. In some embodiments, the present closure is concerned with the generation and utilization of porcine cells in which porcine endogenous retroviral (PERV) elements have been inactivated. In sonic embodiments, the PERV-free or PERV-reduced porcine cells are cloned to produce porcine embryos. In some embodiments, the PERV-free or PERV-reduced embryos may be grown into adult swine from which organs and/or tissues may be extracted and used for such purposes as xenotransplantation into non-porcine animals such as humans. Specification includes a Sequence Listing.
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A method of generating a genetically modified pig comprising inactivated porcine endogenous retrovirus (PERV) pol genes, wherein the porcine endogenous retrovirus (PERV)-inactivated pig comprises somatic pig cells, the method comprising:
A porcine endogenous retrovirus (PERV)-inactivated cell wherein at least 75% of the endogenous PERV pol genes in the cell are inactivated, and wherein inactivation of the PERV pol genes comprises a genetically introduced deletion of greater than 70 base pairs.
A porcine endogenous retrovirus (PERV)-inactivated organ or tissue, comprising porcine cells, wherein at least 75% of the endogenous PERV pol genes in the porcine cells are inactivated, and wherein inactivation of the PERV pol genes comprises a genetically introduced deletion of greater than 70 base pairs.
A porcine endogenous retrovirus (PERV)-inactivated pig, comprising somatic porcine cells, wherein at least 75% of the endogenous PERV pol genes in the somatic porcine cells are inactivated, and wherein inactivation of the PERV pol genes comprises a genetically introduced deletion of greater than 70 base pairs in the endogenous PERV pol genes in the somatic porcine cells.
Dependencies are inferred from the claim text.