US 2025/0040503 A1 · METHOD FOR PRODUCING HAPLOID, AND DOUBLED HAPLOID BETA VULGARIS PLANTS BY MICROSPORE CULTURE

Abstract

The present invention relates to a method for the production of haploid, polyhaploid and/or doubled haploid embryos, calli, seeds and/or plants of the species Beta vulgaris from isolated microspore cultures, more specifically to a method comprising contacting the isolated microspores with a histone deacetylase inhibitor (HDACi) and a complex protein composition. The present invention also provides a kit for producing a haploid, polyhaploid and/or doubled haploid embryo, callus, seed and/or plant of the species Beta vulgaris from at least one isolated microspore as well as the use of a histone deacetylase inhibitor (HDACi) and a complex protein composition for producing a haploid, polyhaploid and/or doubled haploid embryo, callus, seed and/or plant of the species Beta vulgaris. Finally, the present invention also relates to a population of haploid, polyhaploid and/or doubled haploid Beta vulgaris plants directly derived from a single flower, single inflorescence or single bud.

Claims20

Dependent claims

A method for producing a haploid, polyhaploid and/or doubled haploid embryo, callus, seed and/or plant of the species Beta vulgaris comprising:

i) providing at least one isolated microspore of Beta vulgaris,
ii) culturing the at least one microspore from step i) in the presence of a complex protein composition and at least one histone deacetylase inhibitor (HDACi),
iii) culturing the at least one microspore from step ii) to obtain a callus or an embryo, and
iv) optionally, regenerating and thereby obtaining at least one plant from the callus or embryo of step iii), and preferably obtaining at least one haploid, polyhaploid and/or doubled haploid seed thereof.

1 more element

A kit for producing a haploid, polyhaploid and/or doubled haploid embryo, callus, seed and/or plant of the species Beta vulgaris from at least one isolated microspore comprising:

(a) at least one histone deacetylase inhibitor (HDACi), and
(b) a complex protein composition,
wherein the at least one histone deacetylase inhibitor (HDACi) and the complex protein composition are comprised within the same container or within two or more separate containers.

Dependencies are inferred from the claim text.

Method for producing haploid, and doubled haploid beta vulgaris plants by microspore culture

Life of this patent

Method for producing haploid, and doubled haploid beta vulgaris plants by microspore culture

Life of this patent

Abstract

Claims20