A display substrate, comprising a plurality of sub-pixels, wherein at least one sub-pixel comprises a pixel driving circuit and a light-emitting element connected to the pixel driving circuit, the pixel driving circuit comprises a first transistor and a third transistor, the first transistor comprises a first active layer having a first region and a second region, and the third transistor comprises a third active layer located in a semiconductor layer of the pixel driving circuit, a gate electrode of the third transistor is connected to a second node, the pixel driving circuit further comprises a first polar plate, the first polar plate comprises a first portion and a second portion which are of a same material and arranged in a same layer, the first portion serves as the gate electrode of the third transistor, the second portion is connected to the second region of the first transistor via a first connecting electrode.