Display substrate, method for manufacturing the same and display panel

A method for manufacturing a display substrate, wherein the display substrate comprises a plurality of pixel units in a display region, and each of the plurality of pixel units comprises a first thin film transistor, and the method comprises:

• providing a base substrate;
• forming a first semiconductor layer on the base substrate, wherein the first semiconductor layer comprises an active layer of the first thin film transistor, and the active layer of the first thin film transistor at least comprises a channel region and a drain contact region;
• forming an interlayer insulation layer on a side of the first semiconductor layer away from the base substrate;
• and
• forming a through hole in the interlayer insulation layer through a patterning process, and
• forming a first conductive layer on a side of the interlayer insulation layer away from the first semiconductor layer, wherein the first conductive layer comprises a pixel electrode directly and electrically connected to the drain contact region of the active layer of the first thin film transistor through the through hole penetrating through the interlayer insulation layer.