Glucose sensors and methods of use thereof
AssigneeBiocapital Holdings, LLC
InventorsRaul CUERO RENGIFO, Laura Sanchez LONDONO
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Abstract
Described herein are glucose sensors. The sensors are composed of host cells incorporating DNA devices specifically designed to produce fluorescence when the cells come into contact with glucose from a patient sample. Once the fluorescence has been quantified, it can be correlated with the amount of glucose present in the sample. Also described herein are extracts from the host cells that can sense and measure glucose levels in a patient. The devices and extracts disclosed herein are inexpensive but sensitive and accurate enough for use in both home and clinical testing situations. The devices and extracts disclosed herein are also useful for diagnosis of diabetes, pre-diabetes, or other diseases associated with elevated glucose levels.
Claims20
A method for quantifying the amount of glucose in a subject, the method comprising:
- (a) admixing an extract produced by a biological with a sample from the subject to produce a test sample, wherein biological device comprising host cells transformed with a DNA construct, wherein the DNA construct comprises the following genetic components:
- (a) a gene that expresses SNF3, (b) a gene that expresses an O-linked N-acetylglucosamine transferase (OGT), (c) a gene that expresses an O-linked N-acetylglucosamine-selective-N-acetyl-β-D-glucosaminidase (OGlcNa), and (d) optionally a gene that expresses a fluorescent reporter protein;
- (b) measuring the fluorescence produced by the test sample;
- and
- (c) correlating the fluorescence produced by the test sample to the amount of glucose present in the sample.
A method for diagnosing or predicting a disease associated with elevated levels of glucose in a subject, the method comprising the steps of:
- (a) admixing an extract produced by a biological with a sample from the subject to produce a test sample, wherein biological device comprising host cells transformed with a DNA construct, wherein the DNA construct comprises the following genetic components:
- (a) a gene that expresses SNF3, (b) a gene that expresses an O-linked N-acetylglucosamine transferase (OGT), (c) a gene that expresses an O-linked N-acetylglucosamine-selective-N-acetyl-β-D-glucosaminidase (OGlcNa), and (d) optionally a gene that expresses a fluorescent reporter protein;
- (b) measuring the fluorescence produced by the test sample;
- and
- (c) correlating the fluorescence produced by the test sample to the disease.
A glucose sensor comprising an extract produced by a biological with a sample from the subject to produce a test sample, wherein biological device comprising host cells transformed with a DNA construct, wherein the DNA construct comprises the following genetic components:
- (a) a gene that expresses SNF3, (b) a gene that expresses an O-linked N-acetylglucosamine transferase (OGT), (c) a gene that expresses an O-linked N-acetylglucosamine-selective-N-acetyl-β-D-glucosaminidase (OGlcNa), and (d) optionally a gene that expresses a fluorescent reporter protein.
Dependencies are inferred from the claim text.