Method for measuring elastase 1 in feces

Abstract

Provided are an immunoassay reagent and an immunoassay method for elastase 1 (E1) present in human fecal samples, which is simple and can handle a large number of samples without requiring special facilities or equipment, can analyze in a short time so as to be suitable for emergency testing, and can quantitatively analyze a wide range of concentrations from low to high. The immunoassay method, comprising: (a) adding α1-antitrypsin (α1AT) to a fecal sample obtained from a subject; (b) incubating the fecal sample and reacting E1 with α1AT to form a complex; (c) incubating the fecal sample with a solution containing a carrier on which an immunological partner capable of recognizing the E1-α1AT complex is immobilized; and (d) analyzing a change caused by the reaction between the E1-α1AT complex and the immunological partner.

Dependencies are inferred from the claim text.