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US 2025/0035622 A1Published application
Bret-based, dual protease biosensor
AssigneeQatar Foundation for Education, Science and Community Development
InventorsKabir H. Biswas, Asma Fatima, Anupriya M. Geethakumari
Life of this patent
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Abstract
A biosensor for detecting proteolytic cleavage activity of two proteases, coronavirus main protease (Mpro) and papain-like protease (PLpro) is provided. The biosensor utilizes Bioluminescence Resonance Energy Transfer (BRET) between a donor and two acceptor moieties.
Claims25
A bioluminescence resonance energy transfer (BRET) sensor comprising a single polypeptide chain comprising:
- 1) a bioluminescent protein, or a mutant thereof;
- 2) a first BRET acceptor sequence comprising a monomeric NeonGreen (mNG) fluorescent protein;
- and
- 3) a second BRET acceptor sequence comprising a red fluorescent protein (RFP);
- wherein the sensor detects activity of two proteins and wherein a first protein cleaves the first acceptor producing a change in green emission and the second protein cleaves the second acceptor producing a change in red emission.
2 more elements
A dual protease sensor for detecting a coronavirus comprising an amino acid construct of SEQ ID NO:
- 1, SEQ ID NO:
- 3, SEQ ID NO:
- 5, or SEQ ID NO:
- 6.
1 more element
A plasmid comprising a nucleotide construct of SEQ ID NO:
- 2, SEQ ID NO:
- 4, SEQ ID NO:
- 6, or SEQ ID NO:
- 8.
1 more element
Dependencies are inferred from the claim text.