Tunable electroosmotic flow polymer coated capillary
AssigneeUniversity of Notre Dame du Lac
InventorsNorman DOVICHI, Zhenbin ZHANG
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Abstract
A surface-confined aqueous reversible addition-fragmentation chain transfer (SCARAFT) polymerization method was developed to coat capillaries for use in capillary zone electrophoresis (CZE). This coating produced an electroosmotic an order of magnitude lower than that of commercial linear polyacrylamide (LPA)-coated capillaries. Coated capillaries were evaluated for bottom-up proteomic analysis using CZE. The very low electroosmotic mobility results in a 200 min separation and improved single-shot analysis. Various types of coatings were prepared by simply changing the functional vinyl monomers in the polymerization mixture.
Claims22
A method of fabricating a diblock copolymer-coated separation capillary comprising:
- a) contacting the inner surface of a fused silica capillary with a chain transfer reagent to provide a covalently modified inner surface of the fused silica capillary;
- b) contacting the modified inner surface with an aqueous mixture of a radical initiator and a first substituted vinyl monomer;
- c) confining the aqueous mixture to the inner surface of the fused silica capillary;
- d) initiating a living radical polymerization by heating or irradiating the aqueous mixture;
- e) contacting the modified inner surface with an aqueous mixture of a radical initiator and a second substituted vinyl monomer;
- and
- f) repeating steps c) and d) to form a diblock copolymer;
- wherein the diblock copolymer-coated separation capillary is thereby fabricated.
Dependencies are inferred from the claim text.