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US 2025/0034625 A1Published application
Analyte detection
AssigneeThe Regents of the University of Michigan
InventorAlexander Johnson-Buck
Life of this patent
Estimating the term…
Abstract
Provided herein is technology relating to analyte detection and particularly, but not exclusively, to compositions, methods, systems, and kits for high-specificity detection of analytes using molecular probes that integrate cumulative repeated binding of probes to the same analyte molecule.
Claims21
1-43. (canceled)
A method of detecting and/or quantifying an analyte, said method comprising:
- a) providing a sample comprising an analyte;
- b) stably binding the analyte to an integrator probe that is specific for the analyte;
- c) providing a plurality of tally probes that are specific for the analyte and that transiently associate with the analyte,
- wherein each tally probe comprises a label;
- d) contacting the integrator probe with the plurality of tally probes, wherein irreversible transfer of the label from the plurality of tally probes to the integrator probe produces a labeled integrator probe comprising multiple labels;
- and irreversible transfer of the label from a tally probe to the integrator probe occurs more rapidly and/or more efficiently when the tally probe and the integrator probe are both associated with the analyte than when the tally probe and/or the integrator probe is/are dissociated from the analyte;
- and
- e) detecting a signal produced by one or more labeled integrator probes comprising multiple labels,
- wherein detecting the signal indicates that the analyte is present in the sample;
- and/or
- wherein the intensity of the signal or quantity of integrator probes producing the signal indicates the concentration or amount of the analyte in the sample.
8 more elements
45-51. (canceled)
64-73. (canceled)
Dependencies are inferred from the claim text.