Heme-containing cell culture media and uses thereof
AssigneeCalysta, Inc.
InventorsCeline Schiff-Deb, Lorraine Joan Giver, Renee Michelle Saville, Lisa Marie Newman, Warren Kwan, Chien-Seng Hwang, Eric Luning
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Abstract
The present disclosure provides culture or fermentation media including a biomass or derivative thereof of a hemoprotein-producing C1 metabolizing non-photosynthetic bacterium, methods of culturing cells or tissue with the culture or fermentation medium, and products produced by the culturing methods including food products, food ingredients, phytoprotective bacterial cell products, and other products of interest such as vitamins, fatty acids, amino acid, carotenoids, etc.
Claims100
A method of cell or tissue culture or fermentation comprising culturing cells or tissue in a culture or fermentation medium that comprises a biomass or derivative thereof of a hemoprotein-producing C1 metabolizing non-photosynthetic bacterium,
- wherein the amount of heme in the biomass or derivative thereof is in the range of from 0.01 to 10 mg/g protein of the biomass or derivative thereof, and/or
- wherein the amount of the biomass or derivative thereof in the culture or fermentation medium is in the range of 0.1 to 50 g/l.
A cell or tissue culture or fermentation medium, comprising biomass or derivative thereof of a hemoprotein-producing C1 metabolizing non-photosynthetic bacterium,
- wherein the amount of heme in the biomass or derivative thereof is in the range of from 0.01 to 10.0 mg/g protein, and/or
- wherein the amount of the biomass or derivative thereof in the culture medium is in the range of from 0.1 to 50 g/l.
The culture or fermentation medium of any one of 40 to 45, wherein the culture or fermentation medium is suitable for algal cell culture and optionally further comprises seawater base.
The culture or fermentation medium of any one of 40 to 45, wherein the culture or fermentation medium is suitable for fungal cell or tissue culture.
A product of non-bacterial cell or tissue culture or fermentation having a heme iron content that is higher than that of a reference product produced using a culture or fermentation medium that does not comprise biomass or derivative thereof of a C1 metabolizing non-photosynthetic bacterium.
Dependencies are inferred from the claim text.