Method for the fermentative production of l-lysine using c. glutamicum strains expressing heterologous nicotinamide nucleotide transhydrogenase pntab
AssigneeEvonik Operations GmbH
InventorsHugo Ramos Vera, Silke Jerrentrup, Patrick Kottenhahn
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Abstract
Certain C. glutamicum strains overexpress genes coding for enzymes having the function of aspartate-semialdehyde dehydrogenase, aspartate aminotransferase, dihydrodipicolinate synthase, dihydrodipicolinate reductase, diaminopimelate decarboxylase, aspartatokinase and diaminopimelate dehydrogenase. The C. glutamicum strains also express NAD(P)(+) transhydrogenase subunit alpha PntA and NAD(P)(+) transhydrogenase subunit beta PntB of Corynebacterium urealyticum. A method is developed for the fermentative production of L-lysine using such C. glutamicum strains.
Claims7
A C. glutamicum strain, comprising:
- overexpressed genes coding for enzymes having the function of aspartate-semialdehyde dehydrogenase, aspartate aminotransferase, dihydrodipicolinate synthase, dihydrodipicolinate reductase, diaminopimelate decarboxylase, aspartatokinase and diaminopimelate dehydrogenase;
- and a gene coding for an NAD(P)(+) transhydrogenase subunit alpha PntA comprising an amino acid sequence with an identity of at least 80% to the amino acid sequences according to SEQ ID NO:
- 7, and a gene coding for the NAD(P)(+) transhydrogenase subunit beta PntB comprising an amino acid sequence with an identity of at least 80% to the amino acid sequences according to SEQ ID NO:
- 8.
Dependencies are inferred from the claim text.