Engineered threonine aldolases and amino acid decarboxylases
AssigneeCodexis, Inc.
InventorsOscar Alvizo, Scott J. Novick, Nandhitha Subramanian, Thierry Schlama
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Abstract
The present invention provides engineered threonine aldolase and amino acid decarboxylase polypeptides useful for the production of the chiral tertiary amino alcohols, as well as polynucleotides, compositions, and methods utilizing these engineered polypeptides.
Claims29
An engineered threonine aldolase polypeptide comprising a polypeptide sequence having at least 80%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more sequence identity to SEQ ID NO:
- 2, or a functional fragment thereof, wherein said engineered threonine aldolase polypeptide comprises at least one substitution or substitution set in said polypeptide sequence, and wherein the amino acid positions of said polypeptide sequence are numbered with reference to SEQ ID NO:
- 2.
(canceled)
An engineered threonine aldolase polypeptide comprising a polypeptide sequence having at least 80%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more sequence identity to SEQ ID NO:
- 158, or a functional fragment thereof, wherein said engineered threonine aldolase polypeptide comprises at least one substitution or substitution set in said polypeptide sequence, and wherein the amino acid positions of said polypeptide sequence are numbered with reference to SEQ ID NO:
- 158.
(canceled)
An engineered amino acid decarboxylase polypeptide comprising a polypeptide sequence having at least 80%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more sequence identity to SEQ ID NO:
- 282, or a functional fragment thereof, wherein said engineered amino acid decarboxylase polypeptide comprises at least one substitution or substitution set in said polypeptide sequence, and wherein the amino acid positions of said polypeptide sequence are numbered with reference to SEQ ID NO:
- 282.
(canceled)
19-21. (canceled)
-28. (canceled)
(canceled)
(canceled)
-35. (canceled)
Dependencies are inferred from the claim text.