Protease-cleavable masked antibodies
AssigneeDaiichi Sankyo Company, Limited
InventorsShota KUDO, Mikihiro ISHIZUKA, Reiko KAMEI, Tomoko TERAUCHI, Kazunori SAEKI
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Abstract
This invention provides a novel masked antibody. The masked antibody is a molecule that binds to a target antigen, which comprises a moiety binding to a target antigen, a first peptide recognizing a target antigen-binding site comprised in such moiety, and a second peptide comprising an amino acid sequence cleaved by a protease, wherein, after the second peptide is cleaved by a protease, the molecule has higher binding intensity to the target antigen, compared with that before it is cleaved.
Claims35
A molecule comprising a moiety [a], a moiety [b], and a moiety [c] indicated below and binding to a target antigen:
- a moiety [a]:
- a moiety binding to the target antigen;
- a moiety [b]:
- a first peptide recognizing a target antigen-binding site included in the moiety [a];
- and
- a moiety [c]:
- a second peptide comprising an amino acid sequence cleaved by a protease localized in the cytoplasm.
A method for identifying a peptide binding to a complementarity determining region (CDR) comprised in an antibody or an antigen-binding fragment of an antibody comprising a step (i) and a step (ii):
- (i) a step of bringing a peptide library comprising a repeat sequence of aromatic amino acid and Pro into contact with the CDR;
- and
- (ii) a step of collecting a peptide which binds to the CDR.
A peptide library comprising a repeat sequence of aromatic amino acid and Pro.
Dependencies are inferred from the claim text.