Micromixer and Method for Concentration Measurement of Unknown Sample

A microfluidic apparatus comprising:

• a substrate including oppositely disposed first and second ends;
• a mixing chamber including a base, the mixing chamber extending into the substrate, the mixing chamber including a plurality of protruding elements, the base of the mixing chamber including a wall corresponding to the shape of the protruding elements;
• a major channel extending from a first aperture at the first end of the substrate toward the second end of the substrate, the major channel including:
• a first portion extending from the first aperture to and in communication with a second portion, the second portion between the protruding elements and the base proximate to the wall, and a third portion in communication with and extending from the second portion, toward the second end of the substrate;
• and,
• an auxiliary channel extending from a second aperture at the first end of the substrate toward the second end of the substrate.