Three dimensional strobo-stereoscopic imaging systems and associated methods
AssigneeThe Texas A&M University System
InventorChaBum Lee
Life of this patent
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Abstract
A method for reconstructing a three-dimensional (3D) of a sample includes displacing an outer surface of the sample at a desired sample, repeatedly activating an ultraviolet stroboscopic illuminator to produce ultraviolet excitation light at a frequency that is based on the speed of the sample, repeatedly activating a pair of laterally spaced cameras of a stereoscopic imaging unit as the outer surface of the sample is displaced to capture a plurality of 2D images of different sections of the outer surface of the sample, and reconstructing a 3D image of the sample from the plurality of 2D images of the different sections captured by the pair of cameras.
Claims20
A three-dimensional (3D) fluorescent strobo-stereoscopic imaging system, the system comprising:
- a drive configured to couple to a sample to be imaged by the system, wherein the drive is configured to displace an outer surface of the sample at a desired speed;
- an ultraviolet stroboscopic illuminator configured to produce ultraviolet excitation light to stroboscopically illuminate the outer surface of the sample when the sample is coupled to the drive;
- a stroboscopic control module coupled to the drive and the stroboscopic illuminator, wherein the stroboscopic control module comprises a processor and a memory device encoded with instructions that, when executed by the processor of the stroboscopic control module, cause the stroboscopic control module to repeatedly activate the stroboscopic illuminator at a frequency that is based on the speed of the sample;
- a stereoscopic imaging unit comprising a pair of laterally spaced cameras orientable in the direction of the outer surface of the sample and configured to capture two-dimensional (2D) images of different sections of the outer surface of the sample as the outer surface of the sample is displaced by the drive;
- and
- an image reconstruction module in signal communication with the stereoscopic imaging unit, wherein the image reconstruction module comprises a processor and a memory device encoded with instructions that, when executed by the processor of the image reconstruction module, cause the image reconstruction module to reconstruct a 3D image of the sample from the 2D images of the different sections of the outer surface of the sample captured by the pair of cameras of the stereoscopic imaging unit.
A three-dimensional (3D) strobo-stereoscopic imaging system, the system comprising:
- a drive configured to couple to a sample to be imaged by the system, wherein the drive is configured to displace an outer surface of the sample at a desired speed;
- a stroboscopic illuminator configured to produce excitation light to stroboscopically illuminate the outer surface of the sample when the sample is coupled to the drive;
- a stroboscopic control module coupled to the drive and the stroboscopic illuminator, wherein the stroboscopic control module comprises a processor and a memory device encoded with instructions that, when executed by the processor of the stroboscopic control module, cause the stroboscopic control module to repeatedly activate the stroboscopic illuminator at a frequency that is based on the speed of the sample;
- a stereoscopic imaging unit comprising a pair of laterally spaced cameras orientable in the direction of the outer surface of the sample and configured to capture two-dimensional (2D) images of different sections of the outer surface of the sample as the outer surface of the sample is displaced by the drive;
- and
- an image reconstruction module in signal communication with the stereoscopic imaging unit, wherein the image reconstruction module comprises a processor and a memory device encoded with instructions that, when executed by the processor of the image reconstruction module, cause the image reconstruction module to:
- reconstruct an initial surface map of the sample from the 2D images;
- segment the initial surface map based on features contained therein to separate a background image from a target image each contained in the initial surface map;
- separately condition the background image and the target image to produce a conditioned background image and a conditioned target image;
- and
- merge the conditioned background image with the conditioned target image to form a final surface map of the sample.
A method for reconstructing a three-dimensional (3D) image of a sample, the method comprising:
- (a) displacing an outer surface of the sample at a desired sample;
- (b) repeatedly activating an ultraviolet stroboscopic illuminator to produce ultraviolet excitation light at a frequency that is based on the speed of the sample;
- (c) repeatedly activating a pair of laterally spaced cameras of a stereoscopic imaging unit as the outer surface of the sample is displaced to capture a plurality of 2D images of different sections of the outer surface of the sample;
- and
- (d) reconstructing a 3D image of the sample from the plurality of 2D images of the different sections captured by the pair of cameras.
Dependencies are inferred from the claim text.