Transmission electron microscopy with square beams
AssigneeNew York Structural Biology Center
InventorsEugene Yue Dao Chua, Lambertus Michael Alink
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Abstract
An optical system of a Transmission Electron Microscope (TEM) is configured to use a square-shaped electron beam. Preferably, the square-shaped electron beam is produced by using an aperture with a square hole positioned in an aperture plane of TEM's beam shaping aperture (typically, the C2 lens). The square beam enables exhaustive tiling and data collection, enabling the complete imaging of large biological objects. In single particle analysis, a square beam also speeds up data collection rates. These improvements come with no significant loss in imaging quality compared to the standard round beam method of imaging.
Claims14
A method of illuminating a sample in a Transmission Electron Microscope (TEM), the TEM having an illumination system comprising a set of optical lenses arranged in a column, and a camera, comprising:
- configuring the illumination system with a non-circular beam shaping aperture;
- during an illumination mode, illuminating a specimen using a non-circular shaped beam formed by the non-circular beam shaping aperture;
- and
- tiling the non-circular shaped beam to maximize an area of the specimen imaged by the TEM;
- wherein the non-circular beam is rotated with respect to an orientation of the camera.
A method of illuminating a sample in a Transmission Electron Microscope (TEM), the TEM having an illumination system comprising a set of optical lenses arranged in a column, and a camera, comprising:
- configuring the illumination system with a non-circular beam shaping aperture;
- during an illumination mode, illuminating a specimen using a non-circular shaped beam formed by the non-circular beam shaping aperture;
- tiling the non-circular shaped beam to maximize an area of the specimen imaged by the TEM;
- and
- applying fringe-free imaging while illuminating the specimen using the square beam;
- wherein the non-circular beam shaping aperture and the non-circular shaped beam are square;
- wherein the square beam is condensed to the size of the camera.
(canceled)
A Transmission Electron Microscope (TEM), comprising:
- an electron source;
- an illumination system comprising a set of optical lenses arranged in a column, and a camera, the illumination system including a non-circular shaped beam shaping aperture;
- and
- a control system comprising hardware and software, the software configured to control the electron source and the illumination system to illuminate a specimen with a non-circular shaped beam, to rotate the non-circular shaped beam to be square onto the camera, and to tile the non-circular shaped beam across the specimen.
A method operating in a cryo-transmission electron microscope having a camera, comprising:
- illuminating a biological sample using a square shape beam and a fringe-free imaging setup;
- and
- as the biological sample is illuminated, rotating the square shape beam to be square onto the camera.
Dependencies are inferred from the claim text.