Genotyping of cell-free dna from embryo culture media
AssigneeVytelle, LLC
InventorsTasha Gruhot, Bruno Valente Sanches
Life of this patent
Estimating the term…
Abstract
The present disclosure provides, inter alia, techniques, technologies, and methods for non-invasive genotyping and/or embryo sexing, which methods include obtaining media containing cell-free DNA sluffed from an embryo, amplifying the cell-free DNA, and determining the genotype and/or sex of the embryo. In further aspects, the present disclosure provides methods for obtaining estimated breeding values from embryos non-invasively. Also provided are amplification processes to determine embryo genotype including sex.
Claims27
A method for genotyping an organism through cell-free DNA analysis from embryo culture media, comprising the steps of:
- collecting a sample of embryo culture media containing cell-free DNA;
- amplifying the cell-free DNA from the sample;
- and sequencing the amplified cell-free DNA to determine the genotype of the organism.
A device for genotyping an organism through cell-free DNA analysis from embryo culture media, comprising:
- a collection chamber for collecting a sample of embryo culture media containing cell-free DNA;
- an amplification unit for amplifying the cell-free DNA from the sample;
- and a sequencing unit for sequencing the cell-free DNA to determine the genotype of the organism.
A system for genotyping an organism through cell-free DNA analysis from embryo culture media, comprising:
- a collection device for collecting a sample of embryo culture media containing cell-free DNA;
- an amplification unit for amplifying the cell-free DNA from the sample;
- and a sequencing unit for sequencing the cell-free DNA to determine the genotype of the organism;
- wherein the system is capable of generating a genomic profile of the organism.
A computer-implemented method for genotyping an organism through cell-free DNA analysis from embryo culture media, comprising the steps of:
- collecting a sample of embryo culture media containing cell-free DNA;
- amplifying the cell-free DNA from the sample;
- sequencing the cell-free DNA;
- and comparing the sequence data to a reference genome to determine the genotype of the organism.
A kit for genotyping an organism through cell-free DNA analysis from embryo culture media, comprising:
- a collection device for collecting a sample of embryo culture media containing cell-free DNA;
- an amplification unit for amplifying the cell-free DNA from the sample;
- a sequencing unit for sequencing the cell-free DNA;
- and a computer program for comparing the sequence data to a reference genome to determine the genotype of the organism.
(canceled)
(canceled)
A method for non-invasive embryo sexing of an organism through cell-free DNA analysis from embryo culture media. comprising the steps of:
- collecting a sample of embryo culture media containing cell-free DNA;
- amplifying the cell-free DNA from the sample;
- utilizing sex specific primers through PCR to bind to the cell-free DNA in the SCM, and determining the sex of the embryo.
A device for non-invasive embryo sexing of an organism through cell-free DNA analysis from embryo culture media. comprising:
- a collection chamber for collecting a sample of embryo culture media containing cell-free DNA;
- an amplification unit for amplifying the cell-free DNA from the sample utilizing sex specific primers through PCR to bind to the cell-free DNA in the SCM;
- and a computer processor to assess cell free-DNA amplification and primer binding to determine the sex of the embryo through the SCM.
A computer-implemented system for non-invasive embryo sexing of an organism through cell-free DNA analysis from embryo culture media, comprising:
- a collection chamber for collecting a sample of embryo culture media containing cell-free DNA;
- an amplification unit for amplifying the cell-free DNA from the sample utilizing sex specific primers through PCR to bind to the cell-free DNA in the SCM;
- and a computer processor to assess cell free-DNA amplification and primer binding to determine the sex of the embryo through the SCM.
A system for non-invasive embryo sexing of an organism through cell-free DNA analysis from embryo culture media, comprising the steps of:
- collecting a sample of embryo culture media containing cell-free DNA;
- amplifying the cell-free DNA from the sample;
- utilizing sex specific primers through PCR to bind to the cell-free DNA in the SCM, and determining the sex of the embryo.
Dependencies are inferred from the claim text.