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US 2025/0029674 A1Published application
Compositions and methods for expressing genes of interest in host cells
AssigneeNobell Foods, Inc.
InventorsViviane LANQUAR, Leyla HATHWAIK, Yaxin WANG
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Abstract
Provided herein are compositions and methods for stabilizing RNA, increasing protein expression, and combinations thereof. Also provided are compositions and methods for utilizing stabilized RNA or increased protein levels to generate chordate proteins in a host cell, such as a plant cell.
Claims20
A method for selecting a nucleic acid sequence, said method comprising the steps of:
- a) providing data on a plurality of nucleic acid sequences;
- b) predicting secondary structure of the plurality of nucleic acid sequences with a plurality of RNA folding models, such that each nucleic acid sequence in the plurality of nucleic acid sequences is associated with at least two predicted secondary structures;
- c) determining a structural similarity score for the at least two predicted secondary structures associated with each nucleic acid sequence;
- and
- d) selecting a nucleic acid sequence with a higher structural similarity score than at least one other nucleic acid sequence in the plurality of nucleic acid sequences;
- wherein the selected nucleic acid sequence is predicted to accumulate at higher levels when expressed in a host cell.
3 more elements
A method of manufacturing a nucleic acid, said method comprising:
- a) manufacturing a selected nucleic acid sequence to produce a nucleic acid,
- wherein the selection of the nucleic acid sequence was based on the selected nucleic acid sequence having a higher structural similarity score than at least one other nucleic acid sequence in a plurality of nucleic acid sequences;
- wherein the structural similarity score is based on the structural similarity between at least two predicted secondary structures for each nucleic acid sequence, the at least two predicted secondary structures produced by different RNA folding models.
Dependencies are inferred from the claim text.