Method and system for multifunctional image preprocessing and analysis of organoids

A system for analyzing a plurality of organoids, the system comprising:

• an imaging device configured to collect a plurality of images for an organoid of the plurality of organoids;
• and
• a non-transitory medium comprising instructions causing a computer to implement an organoid analysis platform, wherein the organoid analysis platform is configured to:
• determine a plurality of features from the plurality of images, wherein the plurality of features comprise a plurality of an organoid area, a total image area, a percentage of the image covered by organoid, a total intensity of organoid, a total intensity of organoid-by-organoid area, or a total intensity of organoid by total image area;
• determine, using the plurality of features from the plurality of images, one or more differentiation factors between the plurality of images, wherein the one or more differentiation factors comprise small molecule factors and growth factors.